Generic relationships and dating of lineages in Winteraceae based on nuclear (ITS) and plastid (rpS16 and psbA-trnH) sequence data
Marquinez X; Lohmann LG; Faria Salatino ML; Salatino A; Gonzalez F
Molecular Phylogenetics and Evolution
Phylogenetic analyses of representative species from the five genera of Winteraceae (Drimys Pseudowintera Takhtajania Tasmannia and Zygogynum s.l.) were performed using ITS nuclear sequences and a combined data-set of ITS + psbA-trnH + rpS16 sequences (sampling of 30 and 15 species respectively). Indel informativity using simple gap coding or gaps as a fifth character was examined in both data-sets. Parsimony and Bayesian analyses support the monophyly of Drimys Tasmannia and Zygogynum s.l. but do not support the monophyly of Belliolum Zygogynum s.s. and Bubbia. Within Drimys the combined data-set recovers two subclades. Divergence time estimates suggest that the splitting between Drimys and its sister clade (Pseudowintera + Zygogynum s.l.) occurred around the end of the Cretaceous; in contrast the divergence between the two subclades within Drimys is more recent (15.5â€“18.5 MY) and coincides in time with the Andean uplift. Estimates suggest that the earliest divergences within Winteraceae could have predated the first events of Gondwana fragmentation.