We assessed changes in floristic composition, diversity and anthropogenic activities in an East African forest; Echuya Central Forest Reserve (ECFR). Using 272 permanent sample plots, comparisons between 2015 and 2021 were made. Twenty-two tree species were recorded with Macaranga capensis being the most dominant. Tree species density increased significantly from 152 stems/ha in 2015 to 306 stems/ha in 2021. Similarly, trees basal area increased significantly from 19 m(2)/ha in 2015 to 38 m(2)/ha in 2021. The bamboo stem density decreased significantly from 11,931 stems/ha in 2015 to 1807 stems/ha in 2021. Shrubs, lianas, vines and herbs' densities did not show significant differences between 2015 and 2021. Furthermore, a number of human activities significantly increased from 22 activities per hectare in 2015 to 83 per hectare in 2021. The Non-Metric Multidimensional Scaling (NMDS) ordination shows that the human activities with the strongest impact on tree species composition and basal areas were fuelwood collection, human trails, livestock grazing and pole cutting. In conclusion, ECFR is floristically poor; secondary forest tree species are replacing the bamboo forest and human activities could be negatively affecting the ECFR flora. We recommend interventions of increasing people's livelihood incomes to decrease their dependence on forest resources.