This work shows the location and study of relic populations of the Eurasian wild grapevine Vitis vinifera ssp. sylvestris (Gmelin) Hegi in the Asturias region (NW of the Iberian Peninsula). The comprehensive study includes the descrip-tion of their habitats, of the main botanical supporters of these lianas and of the parasitic species causing them bi-ological stress, the ampelographic description of the pop-ulations, wine elaboration from bunches of female vines and a genetic study based on 240 nuclear SNP data. The results obtained have allowed to verify the abundance of this genetic resource in the eastern and central areas of the region. The species that support this liana were different depending on the position (alluvial, colluvial or coastal) of the populations. Several ampelographic differences have been confirmed between male and female individuals. The erineum strain of Colomerus vitis (Pagenstecher) (Acari, Eryophidae) and mildews were the most frequent parasitic species found in the populations. Berries presented a low sugar content, and wines a low alcoholic content and a high total polyphenol index and colour intensity. Feral Vitaceae were found accompanying some of the sylvestris popula-tions mainly in riverbanks and colluvial positions. The ge-netic study showed a clear distinction of the sylvestris vines from the most common grape varieties in the region, even though one of the sylvestris has a compatible parent-off-spring relationship with the variety 'Camaraou Noir'. The progressive reduction of this subspecies as a consequence of the human activities, plagues and diseases highlights the importance of regulating its conservation in order to avoid its extinction.