Background and Aims The large distance between peripheral leaf regions and the petiole in large leaves is expected to cause stronger negative water potentials at the leaf apex and marginal zones compared with more central or basal leaf regions. Leaf zone-specific differences in water supply and/or gas exchange may therefore be anticipated. In this study an investigation was made to see whether zonal differences in gas exchange regulation can be detected in large leaves.\n\nMethods The diurnal course of stomatal conductance gs was monitored at defined lamina zones during two consecutive vegetation periods in the liana Aristolochia macrophylla that has large leaves. Local climate and stem water potential were also monitored to include parameters involved in stomatal response. Additionally leaf zonal vein densities were measured to assess possible trends in local hydraulic supply.\n\nKey Results It was found that the diurnal pattern of gs depends on the position within a leaf in A. macrophylla. The highest values during the early morning were shown by the apical region with subsequent decline later in the morning and a further gradual decline towards the evening. The diurnal pattern of gs at the marginal regions was similar to that of the leaf tip but showed a time lag of about 1 h. At the leaf base the diurnal pattern of gs was similar to that of the margins but with lower maximum gs. At the the leaf centre regions gs tended to show quite constant moderate values during most of the day. Densities of minor veins were lower at the margin and tip compared with the centre and base.\n\nConclusions Gas exchange regulation appears to be zone specific in A. macrophylla leaves. It is suggested that the spatial–diurnal pattern of gs expressed by A. macrophylla leaves represents a strategy to prevent leaf zonal water stress and subsequent vein embolism.